Optimasi Waktu Pre-Heating dan Waktu Pulsed Electric Field terhadap Total Mikroba dan Sifat Fisik Susu
Authors
Angky Wahyu Putranto , Anugerah Dany Priyanto , Teti Estiasih , Widyasari Widyasari , Hadi MunarkoDOI:
10.29303/jrpb.v10i1.321Published:
2022-03-24Issue:
Vol. 10 No. 1 (2022): Jurnal Ilmiah Rekayasa Pertanian dan BiosistemKeywords:
pre-heating, pulsed electric field, susu pasteurisasi, waktu optimalArticles
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Abstract
Pulsed electric field (PEF) atau metode kejut listrik tegangan tinggi merupakan salah satu metode pengolahan non-termal yang dapat diaplikasikan untuk membunuh mikroorganisme pada susu segar. Penggunaan PEF untuk pasteurisasi susu dapat dikombinasikan dengan perlakuan pemanasan awal (pre-heating) untuk meningkatkan letalitas terhadap mikroorganisme maupun untuk menghasilkan susu dengan karakteristik mutu yang diinginkan. Penelitian ini bertujuan untuk melakukan optimasi waktu pre-heating dan waktu PEF pada pengolahan susu pasteurisasi. Optimasi dilakukan dengan menggunakan metode response surface methodology model central composite design (RSM-CCD). Susu segar dengan volume 2,5 L dipanaskan pada suhu 70°C selama 10-30 menit (perlakuan pre-heating) dilanjutkan dengan pemberian kejut listrik (15 kV/cm, 8,197 kHz, 66 µs) selama 2-6 menit. Proses pasteurisasi dilakukan di dalam bejana berpengaduk dengan kecepatan 50 rpm. Respon yang diuji terdiri dari total cemaran (TPC), viskositas, stabilitas emulsi, dan tingkat kecerahan. Hasil optimasi waktu pasteurisasi yang direkomendasikan berdasarkan analisis RSM-CCD, yaitu pre-heating selama 10 menit dilanjutkan dengan PEF selama 3,907 menit dengan nilai desirability 0,614. Optimasi proses pasteurisasi menghasilkan prediksi nilai TPC, viskositas, stabilitas emulsi, dan kecerahan susu hasil optimasi secara berurutan sebesar 2,126 log cfu/ml, 4,997 cP, 14,862 %, dan 93,703.
References
Andriawan, V., & Susilo, B. (2015). “Susu Listrik” Alat Pasteurisasi Susu Kejut Listrik Tegangan Tinggi (Pulsed Electric Field) Menggunakan Transformator Tegangan Tinggi dan Inverter. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 3(2), 199–210.
Angelidis, A. S., Tsiota, S., Pexara, A., & Govaris, A. (2016). The microbiological quality of pasteurized milk sold by automatic vending machines. Letters in Applied Microbiology, 62(6), 472–479. https://doi.org/10.1111/lam.12572.
Braun, K., Hanewald, A., & Vilgis, T. A. (2019). Milk emulsions: Structure and stability. Foods, 8(10), 1–16. https://doi.org/10.3390/foods8100483.
Browning, E., Lewis, M., & Macdougall, D. (2001). Predicting safety and quality parameters for UHT-processed milks. International Journal of Dairy Technology, 54, 111–120.
BSN. (1995). SNI 01-3951-1995: Susu pasteurisasi. Jakarta: Badan Standardisasi Nasional.
Chugh, A., Khanal, D., Walkling-Ribeiro, M., Corredig, M., Duizer, L., & Griffiths, M. W. (2014). Change in color and volatile composition of skim milk processed with pulsed electric field and microfiltration treatments or heat pasteurization. Foods, 3(2), 250–268. https://doi.org/10.3390/foods3020250
Dunkerley, J. A., Ganguli, N. C., & Zadow, J. G. (1993). Reversible changes in the colour of skim milk on heating suggest rapid reversible heat-induced changes in casein micelle size. Australian Journal of Dairy Technology, 48, 66–70.
Hawa, L. C., Susilo, B., & Jayasari, N. E. (2011). Studi Komparasi Inaktivasi Escherichia Coli dan Perubahan Sifat Fisik Pada Pasteurisasi Susu Sapi Segar Menggunakan Metode Pemanasan dan Tanpa Pemanasan dengan Kejut Medan Listrik. Jurnal Teknologi Pertanian, 12(1), 31–39.
Hendrawan, Y., Susilo, B., Putranto, A. W., Riza, D. F. Al, Maharani, D. M., & Amri, M. N. (2016). Optimasi dengan Algoritma RSM-CCD pada Evaporator Vakum Waterjet dengan Pengendali Suhu Fuzzy pada Pembuatan Permen Susu. Jurnal Agritech, 36(02), 226.
Maitimu, C. V, Legowo, A. M., & Al-Baarri, A. N. (2013). Karakteristik Mikrobiologis, Kimia, Fisik dan Organoleptik Susu Pasteurisasi dengan Penambahan Ekstrak Daun Aileru (Wrightia calycina) Selama Penyimpanan. Jurnal Aplikasi Teknologi Pangan, 2(1), 18–29.
Mittal, S., & Bajwa, U. (2014). Effect of heat treatment on the storage stability of low calorie milk drinks. Journal of Food Science and Technology, 51(9), 1875–1883. https://doi.org/10.1007/s13197-012-0714-z
Muslim, C., Hawa, L. C., & Argo, B. D. (2013). Pasteurisasi Non-Termal Pada Susu Sapi Segar untuk Inaktivasi Bakteri Staphylococcus aureus Berbasis Pulse Electric Field ( PEF ). Jurnal Keteknikan, 1(1), 35–49.
Naghili, H., Tajik, H., Mardani, K., Rouhani, S. M. R., Ehsani, A., & Zare, P. (2013). Validation of Drop Plate Technique for Bacterial Enumeration by Parametric and Nonparametric Tests. Veterinary Research Forum, 4(3), 179–183.
Palupi, N. S., Indrastuti, N. A.,Uju, & Syamsir, E. (2020). Optimasi Penggunaan Karagenan dan Kalsium Sulfat pada Pembuatan Tahu Sutra dalam Penembangan Pangan Fungsional. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(2), 272–285. https://doi.org/10.17844/jphpi.v23i2.30973.
Priyanto, A. D., Wicaksono, L. A., & Putranto, A. W. (2021). Pengaruh Suhu dan Waktu Pre-Heating pada Kualitas Fisik , Total Mikroba dan Organoleptik Susu Kolagen Sapi yang Dipasteurisasi. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 9(2), 141–153.
Putranto, A. W., Abida, S. H., Adrebi, K., & Harianti, A. (2020). Lignocellulosic Analysis of Corncob Biomass by Using Non-Thermal Pulsed Electric Field-NaOH Pretreatment. Reaktor, 20(4), 183–191.
Quigley, L., McCarthy, R., O’Sullivan, O., Beresford, T. P., Fitzgerald, G. F., Ross, R. P., … Cotter, P. D. (2013). The microbial content of raw and pasteurized cow milk as determined by molecular approaches. Journal of Dairy Science, 96(8), 4928–4937. https://doi.org/10.3168/jds.2013-6688
Sarkar, S. (2015). Microbiological considerations: Pasteurized milk. International Journal of Dairy Science, 10(5), 206–218. https://doi.org/10.3923/ijds.2015.206.218
Sharma, P., Bremer, P., Oey, I., & Everett, D. W. (2014). Bacterial Inactivation in Whole Milk Using Pulsed Electric Field Processing. International Dairy Journal, 35(1), 49–56.
Sharma, P., Oey, I., Bremer, P., & Everett, D. W. (2014). Reduction of Bacterial Counts and Inactivation of Enzymes in Bovine Whole Milk Using Pulsed Electric Fields. International Dairy Journal, 39(1), 146–156.
Singh, P., Rao, P. S., Sharma, V., & Arora, S. (2021). Physico-chemical aspects of lactose hydrolysed milk system along with detection and mitigation of maillard reaction products. Trends in Food Science and Technology, 107(September 2020), 57–67. https://doi.org/10.1016/j.tifs.2020.11.030.
Wang, C., Lou, X., & Wang, J. (2016). Fatty Acid Composition and Fat Stability of Raw Milk and Pasteurized Milk from Laoshan Goats. Journal of Agricultural Science, 8(6), 149. https://doi.org/10.5539/jas.v8n6p149.
Wulandari, Z., Taufik, E., & Syarif, M. (2017). Kajian Kualitas Produk Susu Pasteurisasi Hasil Penerapan Rantai Pendingin. Jurnal Ilmu Produksi Dan Teknologi Hasil Peternakan, 5(3), 94–100. https://doi.org/10.29244/jipthp.5.3.94-100.
Zhao, L., Zhang, M., Wang, H., & Devahastin, S. (2020). Effect of Carbon Dots in Combination with Aqueous Chitosan Solution on Shelf Life and Stability of Soy Milk. International Journal of Food Microbiology, 326, 108650.
Zheng, M., Jia, Z. B., & Jiang, J. X. (2014). Emulsifying and foaming properties of soy protein isolates with covalent modification by (-)-epigallocatechin-3-gallate. Advanced Journal of Food Science and Technology, 6(2), 238–240.
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